Analysis Note

ControlCos cell lysate with overexpression of RFP

Application

Research Sub CategoryEpitope Tags

Research CategoryEpitope Tags & General Use

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Since the development of Green fluorescent protein's (GFP) use as a protein tag in chimeric proteins, fluorescent proteins have become widely used and useful biotechnological tools. The isolation of Red Fluorescent proteins have opened up a broader color spectrum for use in research. These proteins can be fused with a wide variety of proteins at either the N- or C-terminus, and have been expressed as fluorescent markers in most cell types. These markers can be used as noninvasive markers in both living organisms and cells.

Immunogen

Epitope: Unknown

Recombinant protein corresponding to sea anenome Red fluorescent protein.

Physical form

Unpurified mouse monoclonal ascites with 0.05% sodium azide.

Unpurified

Quality

Evaluated by Western Blot in Cos cell lysate with overexpression of RFP.

Western Blot Analysis: 1:1,000 dilution of this antibody detected Red fluorescent protein on 10 µg of Cos cell lysate with overexpression of RFP.

Specificity

This antibody recognizes Red fluorescent protein.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~ 28 kDa